Name : Mouse R spondin 1/RSPO1 Protein
Product Source :
Recombinant Mouse R spondin 1/RSPO1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser21-Gln265.[Accession | Q9Z132]
Molecular Weight :
The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse R spondin 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Mouse R spondin 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA. Cell Based Assay Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0 – 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).
Background :
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated ‘thrombin-sensitive protein.
Synonyms :
R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1
References & Citations :
(1)Kamata T, Katsube K, Michikawa M, Yamada M, Takada S, Mizusawa H: R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 2004, 1676: 51-62.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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