Name : Human S100A9/MRP14 Protein
Product Source :
Recombinant Human S100A9/MRP14 Protein is expressed from E.coli with His tag at the C-Terminus. It contains Thr2-Pro114.[Accession | P06702]
Molecular Weight :
The protein has a predicted MW of 14.3 kDa.The protein migrates to 27 kDa (dimer)&14.3 kDa (monomer) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human S100A9/MRP14 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human S100A9/MRP14 is greater than 95% as determined by SEC-HPLC.
Background :
S100A8 and S100A9 (also known as MRP8 and MRP14, respectively) are Ca2 binding proteins belonging to the S100 family. They often exist in the form of heterodimer, while homodimer exists very little because of the stability. S100A8/A9 is constitutively expressed in neutrophils and monocytes as a Ca2 sensor, participating in cytoskeleton rearrangement and arachidonic acid metabolism.
Synonyms :
S100-A9; Calgranulin-B; Calprotectin L1H subunit; MRP-14; p14; CAGB; MRP14
References & Citations :
(1) Wang S, Song R, Wang Z, Jing Z, Wang S, Ma J. S100A8/A9 in Inflammation. Front Immunol. 2018 Jun 11;9:1298. doi: 10.3389/fimmu.2018.01298. PMID: 29942307; PMCID: PMC6004386.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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