Name : SARS Spike S1 Protein
Product Source :
Recombinant SARS spike S1 protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Ser14-Arg667.[Accession | P59594]
Molecular Weight :
The protein has a predicted MW of 100.6 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE SARS Spike S1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of SARS spike S1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized SARS Spike S1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, His Tag with the EC50 of 0.21μg/ml determined by ELISA.
Background :
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Synonyms :
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
References & Citations :
(1)Belouzard S , Chu V C , Whittaker G R. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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