Name : Human SECTM1 Protein
Product Source :
Recombinant Human SECTM1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln29-Gly145.[Accession | AAH17716]
Molecular Weight :
The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 47-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human SECTM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human SECTM1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Background :
SECTM1 is a T/NK cell “co-stimulatory” molecule that is expressed in the peripheral blood by neutrophils and monocytes.Human monocytic cells also displayed a pronounced negative regulation of SECTM1 mRNA expression by LPS, while at the protein level SECTM1 expression was also shown to be regulated by IFN and LPS. This tight regulation of SECTM1 gene expression and rapid upregulation highlights its relevance in the innate immune response.
Synonyms :
K-12; SECTM1
References & Citations :
(1) Huyton T, Göttmann W, Bade-Döding C, Paine A, Blasczyk R. The T/NK cell co-stimulatory molecule SECTM1 is an IFN “early response gene” that is negatively regulated by LPS in human monocytic cells. Biochim Biophys Acta. 2011 Dec;1810(12):1294-301. doi: 10.1016/j.bbagen.2011.06.020. Epub 2011 Jul 1. PMID: 21749909.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
VEGF164 Protein
BDNF Protein
Popular categories:
CD43
EphA10