Name : Human SETD7 Protein
Product Source :
Recombinant Human SETD7 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Met1-Lys366.[Accession | NP_085151.1]
Molecular Weight :
The protein has a predicted MW of 41.68 kDa. The protein migrates to 47-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human SETD7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human SETD7 is greater than 95% as determined by SEC-HPLC.
Background :
SETD7 is a methyltransferase that specifically catalyzes the monomethylation of lysine 4 on histone H3. A variety of studies has revealed the role of SETD7 in posttranslational modifications of non-histone proteins. Aberrant expression of SETD7 has been associated with various diseases, including cancer. As a prognostic marker of breast cancer and a novel antioxidant promoter under oxidative stress in breast cancer, SETD7 is considered a good target for the development of new epigenetic drugs.
Synonyms :
KIAA1717; KMT7; SET7; SET9; SET7/9
References & Citations :
Lenstra DC, Damen E, Leenders RGG, Blaauw RH, Rutjes FPJT, Wegert A, Mecinović J. Structure-Activity Relationship Studies on (R)-PFI-2 Analogues as Inhibitors of Histone Lysine Methyltransferase SETD7. ChemMedChem. 2018 Jul 18;13(14):1405-1413. doi: 10.1002/cmdc.201800242. Epub 2018 Jun 25. PMID: 29869845.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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