Name : Human P-Selectin/CD62P Protein
Product Source :
Recombinant Human P-Selectin/CD62P Protein is expressed from HEK293 with His tag at the C-terminus. It contains Trp42-Ala771.[Accession | P16109]
Molecular Weight :
The protein has a predicted MW of 81.05 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human P-Selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human P-Selectin is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human P-Selectin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Background :
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
Synonyms :
FLJ45155; GMP140; GRMP; PADGEM; PSEL; P-Selectin; SELP; CD62P; CD62; LECAM3
References & Citations :
(1)Ataga K I , Kutlar A , Kanter J , et al. Crizanlizumab for the Prevention of Pain Crises in Sickle Cell Disease[J]. New England Journal of Medicine, 2016, 376(5):429-439.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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