Name : Mouse SEMA7A Protein
Product Source :
Recombinant Mouse SEMA7A Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln45-Ala646.[Accession | Q9QUR8]
Molecular Weight :
The protein has a predicted MW of 69.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse SEMA7A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Semaphorin7A (Sema7A) plays an important role in the immunoregulation of the brain.Sema7A is upregulated in the epileptic brain and plays a potential role in the regulation of seizure activity in PTZ-kindled epileptic rats, which may be related to neuroinflammation. Sema7A promotes the inflammatory cytokines TNF-α and IL-6 as well as the growth of mossy fibers through the ERK pathway, suggesting that Sema7A may promote seizures by increasing neuroinflammation and activating pathological neural circuits. Sema7A plays a critical role in epilepsy and could be a potential therapeutic target for this neurological disorder.
Synonyms :
Semaphorin-7A; Semaphorin-K1; Semaphorin-L; Sema L; CD108; CD108MGC126696; CDw108; H-SEMA-K1; H-Sema-L; JMH; MGC126692; Sema7A; SEMAK1; SEMAL
References & Citations :
(1) Deng J, Xu T, Yang J, Zhang KM, Li Q, Yu XY, Li R, Fu J, Jiang Q, Ma JX, Chen YM. Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats. CNS Neurosci Ther. 2020 Jan;26(1):101-116. doi: 10.1111/cns.13181. Epub 2019 Jun 9. PMID: 31179640; PMCID: PMC6930824.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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