Name : Human SIGIRR Protein
Product Source :
Recombinant Human SIGIRR Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Met1-His118.[Accession | Q6IA17-1]
Molecular Weight :
The protein has a predicted MW of 39.29 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human SIGIRR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human SIGIRR is greater than 95% as determined by SEC-HPLC.
Background :
TIR8, also known as single Ig IL-1-related receptor, is a member of the IL-1 receptor/Toll-like receptor (TLR) superfamily, which acts as an intracellular decoy for components of the signaling pathway. Structurally, it is characterized by a single extracellular Ig domain, an intracellular TIR domain and 95-aa cytoplasmic tail. Recently, TIR8 was shown to inhibit NF-_x005f_x0001_B activation by members of the IL-1/TLR family. The inhibitory activity of TIR8 was associated with trapping of TRAF6 and IRAK1.
References & Citations :
(1) Garlanda C, Riva F, Polentarutti N, Buracchi C, Sironi M, De Bortoli M, Muzio M, Bergottini R, Scanziani E, Vecchi A, Hirsch E, Mantovani A. Intestinal inflammation in mice deficient in Tir8, an inhibitory member of the IL-1 receptor family. Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3522-6. doi: 10.1073/pnas.0308680101. Epub 2004 Mar 1. PMID: 14993616; PMCID: PMC373495.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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