Name : Human Sonic Hedgehog (Shh) Protein
Product Source :
Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag. It contains Cys24-Gly197.[Accession | Q15465]
Molecular Weight :
The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Sonic Hedgehog (Shh) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC.
Background :
Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages.
Synonyms :
SHH; Sonic Hedgehog; HHG-1; Sonic hedgehog; HLP3; HPE3; SMMCI; TPT; TPTPS
References & Citations :
Hosoya A, Shalehin N, Takebe H, Shimo T, Irie K. Sonic Hedgehog Signaling and Tooth Development. Int J Mol Sci. 2020 Feb 26;21(5):1587. doi: 10.3390/ijms21051587. PMID: 32111038; PMCID: PMC7084732.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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