Name : Biotinylated Cynomolgus Siglec-15/CD33L3 Protein
Product Source :
Recombinant Biotinylated Cynomolgus Siglec-15/CD33L3 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus. It contains Met60-Thr322.[Accession | XP_005586866.2]
Molecular Weight :
The protein has a predicted MW of 29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl, 0.1M L-arginine (pH 6.0).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Cynomolgus Siglec-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Cynomolgus Siglec-15 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-Siglec-15 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus Siglec-15, His Tag with the EC50 of 13.2ng/ml determined by ELISA.
Background :
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Synonyms :
CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
References & Citations :
(1)Jun Wang,et al.Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy.Nature Medicine (2019).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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