Name : Biotinylated Human Siglec-2/CD22 Protein

Product Source :
Recombinant Biotinylated Human Siglec-2/CD22 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp20-Arg687.[Accession | P20273-1]

Molecular Weight :
The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Human Siglec-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.. SEC-HPLC The purity of Biotinylated Human Siglec-2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human Siglec-2, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.

Background :
CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer’s patches in mice.

Synonyms :
CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814

References & Citations :
(1)Kato J , Robert T. O’Donnell, Abuhay M , et al. Efficacy and toxicity of a CD22-targeted antibody-saporin conjugate in a xenograft model of non-Hodgkin’s lymphoma[J]. OncoImmunology, 2012, 1(9):7.

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