Name : Biotinylated Human Siglec-4a/MAG Protein
Product Source :
Recombinant Biotinylated Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly20-Pro516.[Accession | P20916]
Molecular Weight :
The protein has a predicted MW of 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Siglec-4a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human Siglec-4a is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-Siglec-4a Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 24.4ng/ml determined by ELISA.
Background :
Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).
References & Citations :
(1)Macauley M S , Crocker P R , Paulson J C. Siglec-mediated regulation of immune cell function in disease[J]. Nature Reviews Immunology, 2014, 14(10):653-666.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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