Name : Human SKP1 Protein
Product Source :
Recombinant Human SKP1 Protein is expressed from HEK293 with His tag at the N-terminus and Avi tag at the C-terminus It contains Pro2-Lys163.[Accession | P63208-1]
Molecular Weight :
The protein has a predicted MW of 21.43 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human SKP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human SKP1 is greater than 95% as determined by SEC-HPLC.
Background :
The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes.
Synonyms :
p19A; OCP-2; OCP-II; SIII; EMC19; OCP2; SKP1A; TCEB1L
References & Citations :
Thompson LL, Rutherford KA, Lepage CC, McManus KJ. Aberrant SKP1 Expression: Diverse Mechanisms Impacting Genome and Chromosome Stability. Front Cell Dev Biol. 2022 Mar 8;10:859582. doi: 10.3389/fcell.2022.859582. PMID: 35345853; PMCID: PMC8957228.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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