Name : Human SPARC Protein
Product Source :
Recombinant Human SPARC Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala18-Ile303.[Accession | P09486]
Molecular Weight :
The protein has a predicted MW of 33.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human SPARC on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human SPARC is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human SPARC, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.10μg/ml determined by ELISA.
Background :
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
Synonyms :
SPARC; BM-40; ON
References & Citations :
(1) Rosset EM, Bradshaw AD. SPARC/osteonectin in mineralized tissue. Matrix Biol. 2016 May-Jul;52-54:78-87. doi: 10.1016/j.matbio.2016.02.001. Epub 2016 Feb 3. PMID: 26851678; PMCID: PMC5327628.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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