Name : Human CSPG5 Protein

Product Source :
Recombinant Human CSPG5 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val31-Cys423.[Accession | AAQ04774]

Molecular Weight :
The protein has a predicted MW of 42.47 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 94% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human CSPG5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CSPG5 is greater than 95% as determined by SEC-HPLC.

Background :
Chondroitin sulfate proteoglycan 5 (CSPG-5), also known as neuroglycan C, has been previously associated to differentiation since it shapes neurite growth and synapse forming. CSPG-5 expression shifts in brain areas of the default mode network of suicide victims, which may reflect an impact in the pathogenesis of psychiatric diseases or support diagnostic power.

Synonyms :
Neuroglycan C;CALEB;MGC44034;Neuroglycan C;NGC;

References & Citations :
(1)Pintér A, Hevesi Z, Zahola P, Alpár A, Hanics J. Chondroitin sulfate proteoglycan-5 forms perisynaptic matrix assemblies in the adult rat cortex. Cell Signal. 2020 Oct;74:109710. doi: 10.1016/j.cellsig.2020.109710. Epub 2020 Jul 9. PMID: 32653642.

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