Name : Cynomolgus SFRP2 Protein
Product Source :
Recombinant Cynomolgus SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu25-Cys295.[Accession | G8F2Z7]
Molecular Weight :
The protein has a predicted MW of 32.16 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus SFRP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus SFRP2 is greater than 95% as determined by SEC-HPLC.
Background :
As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients.
Synonyms :
sFRP-2; SARP-1; Sarp1; Sdf5; Sfrp2
References & Citations :
(1) Liu X, Fu J, Bi H, Ge A, Xia T, Liu Y, Sun H, Li D, Zhao Y. DNA methylation of SFRP1, SFRP2, and WIF1 and prognosis of postoperative colorectal cancer patients. BMC Cancer. 2019 Dec 12;19(1):1212. doi: 10.1186/s12885-019-6436-0. PMID: 31830937; PMCID: PMC6909551.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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