Name : Human SIRP Beta 1 isoform 3 Protein
Product Source :
Recombinant Human SIRP Beta 1 isoform 3 Proteinis expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu30-Leu371.[Accession | Q5TFQ8-1]
Molecular Weight :
The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human SIRP Beta 1 isoform 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human SIRP Beta 1 isoform 3 is greater than 95% as determined by SEC-HPLC.
Background :
Signal regulatory protein β1 (SIRPB1) is a signal regulatory protein member of the immunoglobulin superfamily and is capable of modulating receptor tyrosine kinase-coupled signaling. Copy number variations at the SIRPB1 locus were previously reported to associate with prostate cancer aggressiveness in patients.SIRPB1 gene amplification and overexpression were detected in prostate cancer specimens. The knockdown of SIRPB1 significantly suppressed cell growth in colony formation assays and cell mobility.
Synonyms :
SIRP-beta-1 isoform 3; SIRPB1; CD172b; DKFZp686A05192; FLJ26614; 9930027N05Rik; SIRP-beta; Sirpb; Sirpb1; Sirpb1a;SIRP-β-1 isoform 3; SIRP-β
References & Citations :
(1) Song Q, Qin S, Pascal LE, Zou C, Wang W, Tong H, Zhang J, Catalona WJ, Dhir R, Morrell M, Balasubramani GK, Lu Y, Wang Z. SIRPB1 promotes prostate cancer cell proliferation via Akt activation. Prostate. 2020 Mar;80(4):352-364. doi: 10.1002/pros.23950. Epub 2020 Jan 6. PMID: 31905248; PMCID: PMC7421598.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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