Name : Biotinylated Human SIRP alpha V6 Protein
Product Source :
Recombinant Biotinylated Human SIRP alpha V6 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Arg370(S105P).[Accession | P78324 variant 6]
Molecular Weight :
The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human SIRP alpha V6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human SIRP alpha V6 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human SIRP alpha V6, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.21/μg/ml determined by ELISA.
Background :
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the “don´t eat me” signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Synonyms :
CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA
References & Citations :
(1) Weiskopf K , Ring A M , Ho C C M , et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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