Name : Mouse SIRP alpha/CD172a Protein
Product Source :
Recombinant Mouse SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys32-Asn373.[Accession | BAA13520.1]
Molecular Weight :
The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse SIRP alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse SIRP alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse SIRP alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26μg/ml determined by ELISA (QC Test). SPR Data Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 μM as determined in SPR assay (Biacore T200).
Background :
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the “don´t eat me” signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Synonyms :
CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA
References & Citations :
(1)Weiskopf K , Ring A M , Ho C C M , et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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