Name : Human IL-1RL1/ST2 Protein
Product Source :
Recombinant Human IL-1RL1/ST2 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Lys19-Phe328.[Accession | Q01638-2]
Molecular Weight :
The protein has a predicted MW of 61.75 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-1RL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-1RL1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-33, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-1RL1, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Background :
IL1RL1(also known as ST2) is a member of the IL1 receptor family and serves as the receptor for IL-33. The ST2 protein has two isoforms including sST2 and the membrane-bound ST2 receptor, referred to as ST2L. Preclinical studies have demonstrated that binding of IL-33 to ST2L elicits a cardioprotective effect. ST2/IL-33 signaling may play an important role in intestinal disease.
Synonyms :
IL1RL1; DER4; ST2; T1; FIT-1; IL33R; ST2L; ST2V; IL-1 R4; IL1R4; Ly84; MGC32623
References & Citations :
[1] Griesenauer B, Paczesny S. The ST2/IL-33 Axis in Immune Cells during Inflammatory Diseases. Front Immunol. 2017 Apr 24;8:475.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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