Name : Human TFPI-2 Protein

Product Source :
Recombinant Human TFPI-2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp23-Lys213.[Accession | NP_006519.1]

Molecular Weight :
The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human TFPI-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. Bioactivity Data Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is

Background :
Tissue factor pathway inhibitor-2 (TFPI-2) has previously been characterized as an endogenous anticoagulant. TFPI-2 is expressed in the vast majority of cells, mainly secreted into the extracellular matrix. Moreover, in sputum from cystic fibrosis patients TFPI-2 C-terminal fragments are generated and found associated with immunoglobulins. Together our data describe a previously unknown host defense mechanism and therapeutic importance of TFPI-2 against invading Gram-negative bacterial pathogens.

Synonyms :
TFPI-2; PP5; TFPI2; REF1; TFPI-2REF1

References & Citations :
(1) Ali MN, Kasetty G, Elvén M, Alyafei S, Jovic S, Egesten A, Herwald H, Schmidtchen A, Papareddy P. TFPI-2 Protects Against Gram-Negative Bacterial Infection. Front Immunol. 2018 Sep 11;9:2072. doi: 10.3389/fimmu.2018.02072. PMID: 30254643; PMCID: PMC6141739.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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