Name : Biotinylated Human B7-H5/Gi24/VISTA Protein
Product Source :
Recombinant Biotinylated Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Phe33-Ala194.[Accession | Q9H7M9]
Molecular Weight :
The protein has a predicted MW of 21.04 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human B7-H5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human B7-H5 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human B7-H5, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Background :
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.
Synonyms :
B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1
References & Citations :
(1)Byers J T , Paniccia A , Kaplan J , et al. Expression of the Novel Costimulatory Molecule B7-H5 in Pancreatic Cancer[J]. Annals of Surgical Oncology, 2014.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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