Name : Human THSD7A Protein
Product Source :
Recombinant Human THSD7A Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala48–Trp1607.[Accession | Q9UPZ6]
Molecular Weight :
The protein has a predicted MW of 176.22 kDa. Due to glycosylation, the protein migrates to 180-240 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human THSD7A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human THSD7A is greater than 95% as determined by SEC-HPLC.
Background :
Thrombospondin type I domain-containing 7A (THSD7A), is a specific autoantigen of adult idiopathic membranous nephropathy (IMN), whose circulating antibody (THSD7A-AB) represents a promising biomarker for diagnosis of IMN.
Synonyms :
Thrombospondin type-1 domain-containing protein 7A;Gm837;KIAA0960;thrombospondin,type I,domain containing 7A
References & Citations :
Liu Y, Zheng S, Ma C, Lian Y, Zheng X, Guan P, Wang B, Gong X, Gao F, Liang L, Xu D. Meta-Analysis of the Diagnostic Efficiency of THSD7A-AB for the Diagnosis of Idiopathic Membranous Nephropathy. Glob Chall. 2020 Sep 6;4(11):1900099. doi: 10.1002/gch2.201900099. PMID: 33163223; PMCID: PMC7607246.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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