Name : Human TIE1 Protein
Product Source :
Recombinant Human TIE1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala22-Gln759.[Accession | P35590-1]
Molecular Weight :
The protein has a predicted MW of 80.93 kDa. Due to glycosylation, the protein migrates to 81-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TIE1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TIE1 is greater than 95% as determined by SEC-HPLC.
Background :
TIE1 is a cell surface protein expressed in endothelial cells. Involved in angiogenesis and lymphangiogenesis, including morphogenesis of lymphatic valves, TIE1 is important for lymphatic system functional integrity.
Synonyms :
TIE1; TIE; JTK14
References & Citations :
Michelini S, Ricci M, Veselenyiova D, Kenanoglu S, Kurti D, Baglivo M, Fiorentino A, Basha SH, Priya S, Serrani R, Krajcovic J, Dundar M, Dautaj A, Bertelli M. TIE1 as a Candidate Gene for Lymphatic Malformations with or without Lymphedema. Int J Mol Sci. 2020 Sep 16;21(18):6780. doi: 10.3390/ijms21186780. PMID: 32947856; PMCID: PMC7555018.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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