Name : Human TIE2 Protein
Product Source :
Recombinant Human TIE2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala23-Leu748.[Accession | AAA61139.1]
Molecular Weight :
The protein has a predicted MW of 82.09 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TIE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TIE2 is greater than 95% as determined by SEC-HPLC. SPR Data Human TIE2, His Tag immobilized on CM5 Chip can bind Human ANGPT2, His Tag with an affinity constant of 2.38 μM as determined in SPR assay (Biacore T200).
Background :
Angiopoietin-1 (Ang-1) is the primary agonist for Tie2 tyrosine kinase receptor (Tie2), and the effect of Ang-1-Tie2 signalling is context-dependent. Deficiency in either Ang-1 or Tie2 protein leads to severe microvascular defects and subsequent embryonic lethality in murine model. Tie2 receptors are expressed in several cell types, including endothelial cells, smooth muscle cells, fibroblasts, epithelial cells, monocytes, neutrophils, eosinophils and glial cells.
Synonyms :
Tie-2; Angiopoietin-1 receptor; TEK; VMCM; VMCM1; CD202b
References & Citations :
Makinde T, Agrawal DK. Intra and extravascular transmembrane signalling of angiopoietin-1-Tie2 receptor in health and disease. J Cell Mol Med. 2008 Jun;12(3):810-28. doi: 10.1111/j.1582-4934.2008.00254.x. Epub 2008 Feb 4. PMID: 18266978; PMCID: PMC4401129.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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