Name : Biotinylated Human B7-H6 Protein

Product Source :
Recombinant Biotinylated Human B7-H6 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp25-Ser262.[Accession | Q68D85]

Molecular Weight :
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Human B7-H6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human B7-H6 is greater than 95% as determined by SEC-HPLC. SPR Data Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human B7-H6, His-Avi Tag with an affinity constant of 0.227 μM as determined in SPR assay (Biacore T200).

Background :
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.

Synonyms :
B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6

References & Citations :
(1)Wu M R , Zhang T , Gacerez A T , et al. B7H6-Specific Bispecific T Cell Engagers Lead to Tumor Elimination and Host Antitumor Immunity[J]. The Journal of Immunology, 2015, 194(11):5305-5311.

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