Name : Human TIM-4 Protein

Product Source :
Recombinant Human TIM-4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu25-Gln314.[Accession | Q96H15-1]

Molecular Weight :
The protein has a predicted MW of 32.43 kDa. Due to glycosylation, the protein migrates to 78-98 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human TIM-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TIM-4 is greater than 95% as determined by SEC-HPLC.

Background :
Tim4 is a transmembrane protein as the phosphatidylserine (PS) receptor, known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed highly in macrophages, and macrophage Tim-4 inhibits inflammation under various conditions of immune activation.

Synonyms :
TIMD-4; TIMD4; TIM-4;TIM4

References & Citations :
Liu W, Bai F, Wang H, Liang Y, Du X, Liu C, Cai D, Peng J, Zhong G, Liang X, Ma C, Gao L. Tim-4 Inhibits NLRP3 Inflammasome via the LKB1/AMPKα Pathway in Macrophages. J Immunol. 2019 Aug 15;203(4):990-1000. doi: 10.4049/jimmunol.1900117. Epub 2019 Jul 1. PMID: 31263038.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CLEC10A/CD301 ProteinSource
Fc gamma RIIIA/CD16a ProteinPurity & Documentation
Popular categories:
Nerve Growth Factor-β (Beta-NGF)
TIMP-2