Name : Human TIMP1 Protein
Product Source :
Recombinant Human TIMP1 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Cys24-Ala207.[Accession | NP_003245.1]
Molecular Weight :
The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TIMP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TIMP1 is greater than 95% as determined by SEC-HPLC.
Background :
Tissue inhibitor of metalloprotease-1 (TIMP-1) is a tissue inhibitor of matrix metalloproteinases (MMPs). It however exerts multiple effects on biological processes, such as cell growth, proliferation, differentiation and apoptosis, in an MMP-independent manner.
Synonyms :
CLGI; EPA; EPATIMP-1; EPO; TPA-S1; TPAS1; TIMP-1; TIMP
References & Citations :
Wang L, Zhang CG, Jia YL, Hu L. Tissue Inhibitor of Metalloprotease-1 (TIMP-1) Regulates Adipogenesis of Adipose-derived Stem Cells (ASCs) via the Wnt Signaling Pathway in an MMP-independent Manner. Curr Med Sci. 2020 Oct;40(5):989-996. doi: 10.1007/s11596-020-2265-2. Epub 2020 Oct 29. PMID: 33123912.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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