Name : Human TIMP1 Protein

Product Source :
Recombinant Human TIMP1 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Cys24-Ala207.[Accession | NP_003245.1]

Molecular Weight :
The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human TIMP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TIMP1 is greater than 95% as determined by SEC-HPLC.

Background :
Tissue inhibitor of metalloprotease-1 (TIMP-1) is a tissue inhibitor of matrix metalloproteinases (MMPs). It however exerts multiple effects on biological processes, such as cell growth, proliferation, differentiation and apoptosis, in an MMP-independent manner.

Synonyms :
CLGI; EPA; EPATIMP-1; EPO; TPA-S1; TPAS1; TIMP-1; TIMP

References & Citations :
Wang L, Zhang CG, Jia YL, Hu L. Tissue Inhibitor of Metalloprotease-1 (TIMP-1) Regulates Adipogenesis of Adipose-derived Stem Cells (ASCs) via the Wnt Signaling Pathway in an MMP-independent Manner. Curr Med Sci. 2020 Oct;40(5):989-996. doi: 10.1007/s11596-020-2265-2. Epub 2020 Oct 29. PMID: 33123912.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CRELD2 ProteinStorage & Stability
IL-1F10/IL-38 ProteinPurity & Documentation
Popular categories:
Prostate Specific Acid Phosphatase (ACPP)
CEA Cell Adhesion Molecule 21