Name : Human TrkB/NTRK2 Protein
Product Source :
Recombinant Human TrkB/NTRK2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys32-His430.[Accession | AAH31835]
Molecular Weight :
The protein has a predicted MW of 45.2 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TrkB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TrkB is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human/Murine/Rat BDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 50.5ng/ml determined by ELISA.
Background :
Neuron differentiation is likely regulated by a combination of transcription and growth factors. Embryonically, most geniculate neuron development is regulated by the growth factor brain derived neurotrophic factor (BDNF). BDNF expression becomes restricted to subpopulations of taste receptor cells with specific functions,the receptor for BDNF, tropomyosin kinase B receptor (TrkB), may also become developmentally restricted to a subset of taste neurons and could be one factor that is differentially expressed across taste neuron subsets.
References & Citations :
(1) Rios-Pilier J, Krimm RF. TrkB expression and dependence divides gustatory neurons into three subpopulations. Neural Dev. 2019 Jan 28;14(1):3. doi: 10.1186/s13064-019-0127-z. PMID: 30691513; PMCID: PMC6350382.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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