Name : Human TNFRSF11B Protein
Product Source :
Recombinant Human TNFRSF11B Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu22-Leu401.[Accession | O00300]
Molecular Weight :
The protein has a predicted MW of 44.71 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TNFRSF11B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human RANKL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). SPR Data Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Background :
Osteoprotegerin (OPG) is a key regulator in bone metabolism, that also has effect in vascular system. Studies suggest that Osteoprotegerin is a critical arterial calcification inhibitor, and is released by endothelial cells as a protective mechanism for their survival in certain pathological conditions, such as diabetes mellitus, chronic kidney disease, and other metabolic disorders.
Synonyms :
Osteoprotegerin; Ocif; Opg; PDB5; TR1;
References & Citations :
Makarović S, Makarović Z, Steiner R, Mihaljević I, Milas-Ahić J. Osteoprotegerin and Vascular Calcification: Clinical and Prognostic Relevance. Coll Antropol. 2015 Jun;39(2):461-8. PMID: 26753467.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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