Name : Human DcR1/TRAILR3 Protein
Product Source :
Recombinant Human DcR1/TRAILR3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr24-Ala236.[Accession | Q05D71]
Molecular Weight :
The protein has a predicted MW of 23.5 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human DcR1/TRAILR3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human DcR1/TRAILR3 is greater than 95% as determined by SEC-HPLC. SPR Data Human DcR1, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).
Background :
The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family, which mediates apoptosis by the extrinsic pathway. Up-regulation of decoy receptors, DcR1 and DcR2, may result in diminished binding of TRAIL to their functional receptors. DcR1 expression occurs in a subset of EC and may contribute to resistance to TRAIL-induced apoptosis.
Synonyms :
CD263; DCR1; DCR1-TNFR; LIT; TRAIL-R3; TRAILR3; TRID; TNFRSF10C
References & Citations :
(1) Tarragona J, Llecha N, Santacana M, Lopez S, Gatius S, Llobet D, Dolcet X, Palomar-Asenjo V, Gonzalez-Tallada FJ, Matias-Guiu X. DcR1 expression in endometrial carcinomas. Virchows Arch. 2010 Jan;456(1):39-44. doi: 10.1007/s00428-009-0855-2. Epub 2009 Nov 20. PMID: 19936781.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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