Name : Human TRAIL R1/DR4/TNFRSF10A Protein
Product Source :
Recombinant Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Pro34-Asn239.[Accession | O00220]
Molecular Weight :
The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TRAIL R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TRAIL R1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TRAIL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA.
Background :
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis selectively via its interaction with the death receptors TRAILR1/DR4 and TRAILR2/DR5 in a wide range of cancers, while sparing normal cells. Despite its tremendous potential for cancer therapeutics, the translation of TRAIL into the clinic has been confounded by TRAIL-resistant cancer populations.
Synonyms :
DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365
References & Citations :
(1)Deng D, Shah K. TRAIL of Hope Meeting Resistance in Cancer. Trends Cancer. 2020 Dec;6(12):989-1001. doi: 10.1016/j.trecan.2020.06.006. Epub 2020 Jul 24. PMID: 32718904; PMCID: PMC7688478.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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