Name : Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein
Product Source :
Recombinant Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Pro34-Asn239.[Accession | O00220]
Molecular Weight :
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human TRAIL R1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human TRAIL R1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TRAIL, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31μg/ml determined by ELISA (QC Test). SPR Data Biotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.315 nM as determined in SPR assay (Biacore T200).
Background :
Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily.The expression of apoptosis-inducing TRAIL-R1 and TRAIL-R2 and of the decoy receptors TRAIL-R3 and TRAIL-R4 was systematically studied in all developmental stages of peripheral B cells isolated from the blood and secondary lymphoid organs. Expression of TRAIL-Rs is modulated along development, with highest levels observed in germinal center B cells.
Synonyms :
DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365
References & Citations :
(1)Staniek J, Lorenzetti R, Heller B, Janowska I, Schneider P, Unger S, Warnatz K, Seidl M, Venhoff N, Thiel J, Smulski CR, Rizzi M. TRAIL-R1 and TRAIL-R2 Mediate TRAIL-Dependent Apoptosis in Activated Primary Human B Lymphocytes. Front Immunol. 2019 Apr 30;10:951. doi: 10.3389/fimmu.2019.00951. PMID: 31114586; PMCID: PMC6503035.D
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
EGF proteinmedchemexpress
AANAT Proteinweb
Popular categories:
Toll-like Receptor 9
PPAR gamma