Name : Human TREM1 Protein

Product Source :
Recombinant Human TREM1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala21-Arg200.[Accession | NP_061113.1]

Molecular Weight :
The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human TREM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TREM1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TREM1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PGLYRP1, hFc Tag with the EC50 of 0.32μg/ml determined by ELISA.

Background :
TREM1 (Triggering Receptor Expressed on Myeloid Cells 1) is a pro-inflammatory receptor expressed by phagocytes, which can also be released as a soluble molecule (sTREM1). The roles of TREM1 and sTREM1 in liver infection and inflammation are not clear.

Synonyms :
CD354; TREM-1

References & Citations :
(1)Replogle JM, Chan G, White CC, Raj T, Winn PA, Evans DA, Sperling RA, Chibnik LB, Bradshaw EM, Schneider JA, Bennett DA, De Jager PL. A TREM1 variant alters the accumulation of Alzheimer-related amyloid pathology. Ann Neurol. 2015 Mar;77(3):469-77. doi: 10.1002/ana.24337. PMID: 25545807; PMCID: PMC4461024.

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