Name : Human TREM2 Protein

Product Source :
Recombinant Human TREM2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains His19-Ser174.[Accession | Q9NZC2-1]

Molecular Weight :
The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human TREM2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human TREM2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). SPR Data Human TREM2, His Tag captured on CM5 Chip via anti-his antibody can bind Human APOE3, hFc Tag with an affinity constant of 0.41 μM as determined in SPR assay (Biacore T200).

Background :
TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.

Synonyms :
PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c

References & Citations :
(1)Ford J W , Mcvicar D W. TREM and TREM-like receptors in inflammation and disease[J]. Current Opinion in Immunology, 2009, 21(1):38-46.

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