Name : Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein
Product Source :
Recombinant Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein is expressed from E.coli without tag. It contains Gln283-Arg396.[Accession | P12643]
Molecular Weight :
The protein has a predicted MW of 12.90 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 100mM Acetic Acid (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 100mM Acetic Acid (pH 2.8).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human/Rhesus macaque/Mouse/Rat/Canine BMP2 on Tris-Bis PAGE under Non reducing (N) condition and reduced condition. The purity is greater than 95%. Cell Based Assay Measured by its ability to induce Alkaline Phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 40-200 ng/mL (QC Test).
Background :
Bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor-β (TGF-β) super-family, is one of the main chondrogenic growth factors involved in cartilage regeneration. BMP2 is known to induce chondrogenic differentiation in various types of stem cells in vitro. However, BMP2 also induces osteogenic differentiation and endochondral ossification in mesenchymal stem cells (MSCs).
Synonyms :
BMP-2; BMP-2A; BMP2A; BDA2; SSFSC;
References & Citations :
Zhou N, Li Q, Lin X, Hu N, Liao JY, Lin LB, Zhao C, Hu ZM, Liang X, Xu W, Chen H, Huang W. BMP2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells. Cell Tissue Res. 2016 Oct;366(1):101-11. doi: 10.1007/s00441-016-2403-0. Epub 2016 Apr 15. PMID: 27083447.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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