Name : Human TSLPR Protein
Product Source :
Recombinant Human TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly25-Lys231.[Accession | Q9HC73-1]
Molecular Weight :
The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TSLPR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TSLPR, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human TSLP, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 1.33μg/ml determined by ELISA.
Background :
TSLP is an epithelial cell-derived cytokine synthesized in response to various stimuli, including protease allergens and microorganisms like viruses and bacteria. Biological functions of TSLP require heterodimer formation between the TSLP receptor (TSLPR) and IL-7 receptor-α, which polarize dendritic cells to induce type 2 inflammation and directly expand and/or activate Th2 cells, group 2 innate lymphoid cells, basophils, and other immune cells.
Synonyms :
TSLP receptor; TSLPR; CRL2; CRLF2Y; CRLF2; IL-XR; p2RY8/CRLF2 fusion; CRLF2
References & Citations :
(1) Nakajima S, Kabata H, Kabashima K, Asano K. Anti-TSLP antibodies: Targeting a master regulator of type 2 immune responses. Allergol Int. 2020 Apr;69(2):197-203. doi: 10.1016/j.alit.2020.01.001. Epub 2020 Jan 21. PMID: 31974038.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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