Name : Human BTN3A3/BTF3 Protein
Product Source :
Recombinant Human BTN3A3/BTF3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln30-Trp248.[Accession | O00478-1]
Molecular Weight :
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human BTN3A3/BTF3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human BTN3A3/BTF3 is greater than 95% as determined by SEC-HPLC.
Background :
The butyrophilin (BTN) family has many members with diverse functions related to immunomodulation, initiation and progression of tumors. BTN3A3 belongs to the BTN family, and exploring its expression and correlation with the prognosis of non-small cell lung cancer (NSCLC) patients has great clinical significance.The expression of BTN3A3 in NSCLC tissues was significantly lower than in adjacent tissues, and patients with low expression of BTN3A3 had late clinical stages and lower degree of tumor differentiation.
Synonyms :
BTN3A3; BTF3; BTN3.3; BTF3butyrophilin 3
References & Citations :
(1) Cheng X, Ma T, Yi L, Su C, Wang X, Wen T, Wang B, Wang Y, Zhang H, Liu Z. Low expression of BTN3A3 indicates poor prognosis and promotes cell proliferation, migration and invasion in non-small cell lung cancer. Ann Transl Med. 2021 Mar;9(6):478. doi: 10.21037/atm-21-163. PMID: 33850875; PMCID: PMC8039694.
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