Name : Human TYRO3 Protein
Product Source :
Recombinant Human TYRO3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala41-Trp429.[Accession | Q06418]
Molecular Weight :
The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TYRO3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TYRO3 is greater than 95% as determined by SEC-HPLC. SPR Data Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 μM as determined in SPR assay (Biacore T200).
Background :
The TAM receptors (TYRO3, Axl and MerTK) are promising therapeutic targets on tumor-associated macrophages. The TAM receptors are a family of receptor tyrosine kinases with shared ligands Gas6 and Protein S that skew macrophage polarization towards a pro-tumor M2-like phenotype.In macrophages, the TAM receptors also promote apoptotic cell clearance, a tumor-promoting process called efferocytosis. The TAM receptors bind the “eat-me” signal phosphatidylserine on apoptotic cell membranes using Gas6 and Protein S as bridging ligands.
Synonyms :
BYK; DTK; RSE; SKY; TIF; TYRO3; Brt; Rse
References & Citations :
(1) Myers KV, Amend SR, Pienta KJ. Targeting TYRO3, Axl and MerTK (TAM receptors): implications for macrophages in the tumor microenvironment. Mol Cancer. 2019 May 14;18(1):94. doi: 10.1186/s12943-019-1022-2. PMID: 31088471; PMCID: PMC6515593.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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