Name : Human ULBP-6 Protein
Product Source :
Recombinant Human ULBP-6 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Arg26-Gly218.[Accession | Q5VY80]
Molecular Weight :
The protein has a predicted MW of 48.61 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ULBP-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ULBP-6 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human NKG2D, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
Background :
ULBP6/RAET1L (UL16-binding protein, also known as retinoic acid early transcript), is expressed as a full-lengthtranscript in cells and encodes a protein that is a ligand for bothNKG2D and the UL16 protein of HCMV.
Synonyms :
RAET1L; ULBP6;
References & Citations :
Eagle RA, Traherne JA, Hair JR, Jafferji I, Trowsdale J. ULBP6/RAET1L is an additional human NKG2D ligand. Eur J Immunol. 2009 Nov;39(11):3207-16. doi: 10.1002/eji.200939502. PMID: 19658097.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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