Name : Mouse VEGF R3/FLT4 Protein
Product Source :
Recombinant Mouse VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Tyr25-Glu775.[Accession | P35917]
Molecular Weight :
The protein has a predicted MW of 111.69 kDa. Due to glycosylation, the protein migrates to 115-160 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse VEGF R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse VEGF R3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse VEGF R3, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). SPR Data Mouse VEGF R3, hFc Tag captured on CM5 Chip via Protein A can bind Human VEGF-C, His Tag with an affinity constant of 12.93 pM as determined in SPR assay (Biacore T200).
Background :
Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.
Synonyms :
VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL
References & Citations :
(1) Okita NT, Yamada Y, Takahari D, Hirashima Y, Matsubara J, Kato K, Hamaguchi T, Shirao K, Shimada Y, Taniguchi H, Shimoda T. Vascular endothelial growth factor receptor expression as a prognostic marker for survival in colorectal cancer. Jpn J Clin Oncol. 2009 Sep;39(9):595-600. doi: 10.1093/jjco/hyp066. Epub 2009 Jun 17. PMID: 19535387.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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