Name : Human BLOC1S2 Protein

Product Source :
Recombinant Human BLOC1S2 Protein is expressed from E.coli without tag. It contains Phe2-Arg99.[Accession | Q6QNY1-2]

Molecular Weight :
The protein has a predicted MW of 11.35 kDa same as Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 150mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human BLOC1S2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human BLOC1S2 is greater than 90% as determined by SEC-HPLC.

Background :
BLOC1S2 (Biogenesis of lysosome-related organelles complex-1 subunit 2) protein is widely expressed in normal tissue as well as in malignant tumors with a tendency towards lower expression levels in certain subtypes of tumors. On the subcellular level, BLOC1S2 is expressed in an organellar-like pattern and co-localizes with mitochondria.

Synonyms :
BLOC-1 subunit 2; BLOS2; CEAP; CEAP11

References & Citations :
Gdynia G, Lehmann-Koch J, Sieber S, Tagscherer KE, Fassl A, Zentgraf H, Matsuzawa S, Reed JC, Roth W. BLOC1S2 interacts with the HIPPI protein and sensitizes NCH89 glioblastoma cells to apoptosis. Apoptosis. 2008 Mar;13(3):437-47. doi: 10.1007/s10495-007-0176-3. PMID: 18188704.

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