Name : Cynomolgus BST2 Protein
Product Source :
Recombinant Cynomolgus BST2 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ile49-Ser163.[Accession | XP_005588438.1]
Molecular Weight :
The protein has a predicted MW of 13.78 kDa. Due to glycosylation, the protein migrates to 15-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus BST2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.
Synonyms :
BST-2; HM1.24 antigen; CD317; BST2; NPC-A-7; PDCA-1; TETHERIN
References & Citations :
(1) Kong N, Shan T, Wang H, Jiao Y, Zuo Y, Li L, Tong W, Yu L, Jiang Y, Zhou Y, Li G, Gao F, Yu H, Zheng H, Tong G. BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy. Autophagy. 2020 Oct;16(10):1737-1752. doi: 10.1080/15548627.2019.1707487. Epub 2019 Dec 27. PMID: 31868081.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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