Name : Cynomolgus ADAM9 Protein

Product Source :
Recombinant Cynomolgus ADAM9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala29-Gly698.[Accession | A0A2K5X4X8]

Molecular Weight :
The protein has a predicted MW of 74.90 kDa. Due to furin cleavage site, the mature form migrates to 65-115 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 90% as determined by Tris-Bis PAGE> 90% as determined by HPLC

Formulation :
Supplied as 0.22μm filtered solution in 50mM Tris, 500mM NaCL, 0.005% Brij-35, 20% Glycerol (pH 8.5).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Cynomolgus ADAM9 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. SEC-HPLC The purity of Cynomolgus ADAM9 is greater than 90% as determined by SEC-HPLC. Bioactivity Data Measured by its ability to cleave a fluorogenic peptidesubstrate Mca-PLAQAV-Dpa-RSSSR-NH The specificactivity is > 1.0 pmol/min/ug.

Background :
A disintegrin and metalloproteinase 9 (ADAM9) is a member of the transmembrane ADAM family. It is expressed in different types of solid cancer and promotes tumor invasiveness. ADAM9 may be a prognostic marker for vestibular schwannomas (VS), and ADAM9 inhibition might have the potential as a systemic approach for the treatment of VS.

Synonyms :
ADAM 9; Meltrin-gamma; Mdc9; Mltng; Adam9; Kiaa0021; AU020942; MDC9; mKIAA0021; CORD9; MCMP; MLTNG

References & Citations :
(1) Breun M, Schwerdtfeger A, Martellotta DD, Kessler AF, Monoranu CM, Matthies C, Löhr M, Hagemann C. ADAM9: A novel player in vestibular schwannoma pathogenesis. Oncol Lett. 2020 Mar;19(3):1856-1864. doi: 10.3892/ol.2020.11299. Epub 2020 Jan 14. PMID: 32194680; PMCID: PMC7039135.

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