Name : Human BTN3A1/CD277 Protein
Product Source :
Recombinant Human BTN3A1/CD277 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln30-Gly254.[Accession | O00481-1]
Molecular Weight :
The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human BTN3A1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human BTN3A1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA.
Background :
The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
Synonyms :
CD277; BTN3A1; BTF5; BT3.1; BTN3.1
References & Citations :
(1) Rhodes DA, Chen HC, Price AJ, Keeble AH, Davey MS, James LC, Eberl M, Trowsdale J. Activation of human γδ T cells by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin. J Immunol. 2015 Mar 1;194(5):2390-8. doi: 10.4049/jimmunol.1401064. Epub 2015 Jan 30. PMID: 25637025; PMCID: PMC4337483.Copy
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
B3GAT1 ProteinMedChemExpress
IL-19 ProteinPurity & Documentation
Popular categories:
VEGF & VEGFR
KIR2DS2