Name : Human CALCA/CGRP Protein
Product Source :
Recombinant Human CALCA/CGRP Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala26-Asn141.[Accession | NP_001732.1]
Molecular Weight :
The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CALCA/CGRP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CALCA/CGRP is greater than 95% as determined by SEC-HPLC.
Background :
Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.
Synonyms :
Calcitonin; CCP; PDN-21; CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; PCT; ProCalcitonin; Calcitonin 1; Katacalcin
References & Citations :
(1) Wang L, Ding F, Shi S, Wang X, Zhang S, Song Y. Hypermethylation in Calca Promoter Inhibited ASC Osteogenic Differentiation in Rats with Type 2 Diabetic Mellitus. Stem Cells Int. 2020 Mar 4;2020:5245294. doi: 10.1155/2020/5245294. PMID: 32190058; PMCID: PMC7073499.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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