Name : Human NCAM-1/CD56 Protein
Product Source :
Recombinant Human NCAM-1/CD56 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu20-Gly718.[Accession | P13591-2]
Molecular Weight :
The protein has a predicted MW of 78.48 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human NCAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human NCAM-1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human NCAM-1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
Background :
Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.
Synonyms :
N-CAM-1; NCAM-1; NCAM1; CD56; NCAM; MSK39
References & Citations :
Guven A, Gray K, Peng KW, Klotz A, Kellogg MD, Narain NR, Kiebish MA. Development and validation of an immunoassay for quantification of NCAM-1 in human plasma. J Pharm Biomed Anal. 2021 Apr 15;197:113981. doi: 10.1016/j.jpba.2021.113981. Epub 2021 Feb 16. PMID: 33657526.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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