Name : Mouse CA2/Carbonic anhydrase II Protein
Product Source :
Recombinant Mouse CA2/Carbonic anhydrase II Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ser2-Lys260.[Accession | P00920]
Molecular Weight :
The protein has a predicted MW of 29.99 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse CA2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CA2 is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its esterase activity. The specific activity is > 100 pmol/min/µg.
Background :
Carbonic anhydrase II (CA II) is a zinc metalloenzyme that catalyzes the reversible interconversion of water and CO2 to bicarbonate and a proton. CA II is abundant in most cells, and plays a role in numerous processes including gas exchange, epithelial ion transport, respiration, extra- and intracellular pH control, and vascular regulation.
Synonyms :
Carbonic anhydrase 2; CA-II; Car2; CA2
References & Citations :
Andring JT, Lomelino CL, Tu C, Silverman DN, McKenna R, Swenson ER. Carbonic anhydrase II does not exhibit Nitrite reductase or Nitrous Anhydrase Activity. Free Radic Biol Med. 2018 Mar;117:1-5. doi: 10.1016/j.freeradbiomed.2018.01.015. Epub 2018 Jan 31. PMID: 29355738.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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