Name : Biotinylated Cynomolgus IL-2 R gamma/CD132 Protein (Primary Amine Labeling)

Product Source :
Recombinant Biotinylated Cynomolgus IL-2 R gamma/CD132 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus. It contains Leu23-Asn254.[Accession | Q38JL2]

Molecular Weight :
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Cynomolgus IL-2 R gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Background :
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling.

Synonyms :
CD132; CIDX; IL-2 R gamma; IL2RG; IMD4; P64; SCIDX; SCIDX1; gammaC

References & Citations :
(1) Smith K A. The structure of IL2 bound to the three chains of the IL2 receptor and how signaling occurs[J]. Medical Immunology, 2006, 5(1).

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