Name : Rhesus macaque CD155/PVR Protein
Product Source :
Recombinant Rhesus macaque CD155/PVR Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp28-Asn343.[Accession | Q0MSE6-1]
Molecular Weight :
The protein has a predicted MW of 35.5 kDa. Due to glycosylation, the protein migrates to 46-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Rhesus macaque CD155/PVR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Rhesus macaque CD155/PVR is greater than 95% as determined by SEC-HPLC. SPR Data Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 μM as determined in SPR assay (Biacore T200).
Background :
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the “poliovirus receptor,” or PVR.
Synonyms :
CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
References & Citations :
(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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